饲料用酶制剂开发与酶工程、蚕桑资源综合利用
发表论文:
[1] Ying-Zhi Peng, Xiao-Lu Zhu, Xiao-Xiao He, Yi-Hao Chen, Xiang Lv, Le-Yun Yang, Jing Li, Wei-Guo Zhao, Jun Wang, Shuai You*. Thermostability improvement of the glucose oxidase from Penicillium amagasakiense for efficient antimicrobial performance through computer-aided molecular design. International Journal of Biological Macromolecules. 284 (2025), 137932. https://doi.org/10.1016/j.ijbiomac.2024.137932.(中科院1区,IF:6.9)
[2] Shuai You, Wen-Xin Zhang, Yan Ge, Yu Lu, Richard Ansah Herman, Yi-Wen Chen, Sheng Zhang, Yang-Hao Hu, Zhi-Yuan Bai, Jun Wang*. Improvement of GH10 xylanase activity based on channel hindrance elimination strategy for better synergistic cellulase to enhance green bio-energy production. Renewable Energy. 2023,215: 118967.(中科院1区,IF:9.0)
[3] Shuai You, Lin-Lin Zhu, Shu-Meng Zhang, Richard-Ansah Herman, Yang Li, Li-Tian Geng, Huan Chen, Fu-An Wu, Jun Wang. Recyclable and efficient isoquercitrin production with whole-cell catalysis of improved GH78 α-L-rhamnosidase by domain truncation strategy. Industrial Crops & Products. 2023, 191:115945. (中科院1区,IF:6.4)
[4] Shuai You, Yi-Xin Zhang, Fan Shi, Wen-Xin Zhang, Jing Li, Sheng Zhang, Zhong-Li Chen, Wei-Guo Zhao, Jun Wang*. Lowering energy consumption for fermentable sugar production from Ramulus mori: Engineered xylanasesynergy and improved pretreatment strategy. Bioresour Technol. 2022, 344(Pt B):126368.(中科院1区,IF:11.9)
[5]Shuai You, Jing Li, Fang Zhang, Zhi-Yuan Bai, Saidi Shittu, Richard-Ansah Herman , Wen-Xin Zhang, Jun Wang*. Loop engineering of a thermostable GH10 xylanase to improve low-temperature catalytic performance for better synergistic biomass-degrading abilities. Bioresour Technol. 2021, 342:125962.(中科院1区,IF:11.9)
[6] Shuai You, Ziqian Zha, Jing Li, Wenxin Zhang, Zhiyuan Bai, Yanghao Hu, Xue Wang, Yiwen Chen, Zhongli Chen, Jun Wang* and Huiying Luo*. Improvement of XYL10C_ΔN catalytic performance through loop engineering for lignocellulosic biomass utilization in feed and fuel industries. Biotechnol Biofuels, 2021, 14:195.(中科院1区,IF:7.7)
[7] Shuai You, Chen Xie, Rui Ma, Huo-qing Huang, Richard Ansah Herman, Xiao-yun Su, Yan Ge, Hui-yi Cai, Bin Yao,Jun Wang* and Hui-ying Luo*. Improvement in catalytic activity and thermostability of a GH10 xylanase and its synergistic degradation of biomass with cellulase. Biotechnol Biofuels. 2019, 12:278.(中科院1区,IF:7.7)
[8] Shuai You, Tao Tu, Rui Ma, Huo-qing Huang, Yuan Wang, Ying-guo Bai, Xiao-yun Su, Hui-yi Cai, Bin Yao,* and Hui-ying Luo*. Functional analysis of a highly active β-glucanase from Bispora sp. MEY-1 using its C-terminally truncated mutant. J Agric Food Chem. 2018, 66(37):9728-9737.(中科院1区,IF:5.8)
[9] Shuai You, Chun-Chi Chen, Tao Tu, Xiaoyu Wang, Rui Ma, Hui-yi Cai, Rey-Ting Guo*, Hui-ying Luo* and Bin Yao*. Insight into the functional roles of Glu175 in the hyperthermostable xylanase XYL10C-ΔN through structural analysis and site-saturation mutagenesis. Biotechnol Biofuels. 2018, 11:159.(中科院1区,IF:7.7)
[10] Shuai You, Tao Tu, Lujia Zhang, Yuan Wang, Huoqing Huang, Rui Ma, Pengjun Shi, Yingguo Bai, Xiaoyun Su, Zhemin Lin, Huiying Luo* and Bin Yao*. Improvement of the thermostability and catalytic efficiency of a highly active β-glucanase from Talaromyces leycettanus JCM12802 by optimizing residual charge-charge interactions. Biotechnol Biofuels. 2016, 9:124.(中科院1区,IF:7.7)
[11] Zhi-Yuan Bai, Shuai You, Fang Zhang, Zhi-Wei Dong, Yi-Fan Zhao, Hong-Jian Wen, Jun Wang. Efficient fermentable sugar production from mulberry branch based on a rational design of GH10 xylanase with improved thermal stability. Renewable Energy, 2023, 206: 566-573.(学生第一,导师第二,中科院1区,IF:9.0)
[12] Fan Zhang, Shuai You, Ting Huang, Jin-Zheng Wang, Lin-Lin Zhu, Bo Wang, Wang-Sheng Ye, Richard Ansah Herman, Heng Luo, Jun Wang⁎. Dual promoter strategy enhances co-expression of α-L-rhamnosidase and enhanced fluorescent protein for whole-cell catalysis and bioresource valorization. Science of the Total Environment. 2020, 722:137865.(学生第一,导师第二,中科院1区,IF:8.0)
授权/发明专利:
[1] 游帅;张温馨;查子千;谢晨;葛研;胡杨浩;王俊. Xylanase mutant and its preparation method and application. 美国发明专利授权, 专利号: US11,447,761 B2, 授权公告日: 2022.09.20.申请日: 2021.02.07.
[2] 游帅;陈忠立;庄愉;储呈平;葛研;王俊. GH10フアミリ-の耐高温キシラナ一ゼ突然変異体群及びその使用(一组 GH10 家族耐高温木聚糖酶突变体及其应用). 专利号: 7449605, 授权公告日: 2024.03.06, 日本发明专利授权(日本专利局), 申请日: 2021.08.20, 申请号: 2022-575276.
[3] 游帅;陈忠立;庄愉;储呈平;葛研;王俊. Group of high temperature resistant xylanase mutants of GH10 family and application thereof. PCT专利申请, PCT/CN 2021/113743, 国际公布日:2021.09.15.
[4] 游帅;张温馨;查子千;谢晨;葛研;胡洋浩;王俊. 木聚糖酶突变体及其制备方法与应用. 专利号: ZL202110178825.2, 授权公告日: 2021.08.10. 申请日: 2021.02.07.
[5] 游帅;葛研;谢晨;查子千;王俊. 一种40°C下高比活木聚糖酶突变体及其构建方法与应用. 专利号: ZL201910970877.6, 授权公告日: 2021.08.27. 申请日: 2019.10.14.
[6] 游帅;张温馨;谢晨;查子千;葛研;王俊. 一种GH16家族耐热葡聚糖酶突变体及其构建方法与应用. 专利号: ZL202011456604.9, 授权公告日: 2021.11.09. 申请日: 2020.12.10.
[7] 游帅;王俊;谢晨;朱琳琳;盛晟;吴福安.一种高催化效率木聚糖酶突变体及其构建方法与应用. 专利号: ZL201910659589.9, 授权公告日: 2022.02.08. 申请日: 2019.07.22(转化5.1万元, 转化日: 2023.06.28)
[8] 游帅;陈忠立;庄愉;储呈平;葛研;王俊. 一组GH10家族耐高温木聚糖酶突变体及其应用. 专利号: ZL202110854745.4, 授权公告日: 2022.07.22. 申请日: 2021.07.28.
[9] 游帅;谢晨;张温馨;张访;陈奕文;胡洋浩;王俊. 一组GH16家族耐热β-1, 3-1, 4-葡聚糖酶突变体及其应用. 专利号: ZL202110623476.0, 授权公告日: 2022.07.22. 申请日: 2021.06.04.
[10] 游帅;查子千;谢晨;张温馨;张访;王雪;陈奕文;王俊. 一组GH16家族葡聚糖酶的N-糖基化突变体及其应用. 专利号: ZL202110621950. 6, 授权公告日: 2022.07.22. 申请日: 2021.06.04.
[11] 葛研;游帅;谢晨;查子千;王俊. 动物体温下高比活耐热木聚糖酶突变体及其应用. 专利号: ZL202110239347.1, 授权公告日: 2022.07.22. 申请日: 2021.03.04.
[12] 游帅;葛研;谢晨;张温馨;王雪;陈奕文;张伊欣;王俊. 一组动物体温下高催化活力β-1,3-1,4-葡聚糖酶突变体及其应用. 专利号: ZL202110629186.7, 授权公告日: 2023.02.03. 申请日: 2021.06.04.
[13] 游帅;胡洋浩;赵卫国;王俊;陈奕文;李龙. 一种耐热木聚糖酶突变体及其应用. 专利号: ZL202111393384.4, 授权公告日: 2023.04.18. 申请日: 2021.11.23.
[14] 游帅;张晟;胡洋浩;张访;张温馨;陈奕文;王俊. 一种高比活木聚糖酶突变体及其应用. 专利号: ZL202111395572.0, 授权公告日: 2023.04.18. 申请日: 2021.11.23.
[15] 游帅;张温馨;陈忠立;周春生;储呈平;孙道权;王俊. 一种葡聚糖酶突变体及其应用. 专利号: ZL202210022750.3, 授权公告日: 2024.02.20. 申请日: 2022.01.10.
[16] 游帅;张温馨;陈忠立;周春生;储呈平;赵卫国;王俊. 热稳定性提升的酸性葡聚糖酶突变体及其应用. 专利号: ZL202210045782.5, 授权公告日: 2024.03.15. 申请日: 2022.01.16.
[17] 游帅;卢宇;张温馨;张伊欣;张颖;白致远;徐远志;王俊. 一种耐热葡萄糖氧化酶突变体及其应用.专利号: ZL202211588610.9, 授权公告日: 2024.12.20, 申请号: 20221588610.9, 申请日: 2022.12.09.
[18] 姚斌;罗会颖;游帅;王苑;涂涛;黄火清;苏小运;伯映国;王亚茹. 一种木聚糖酶突变体及其制备方法和应用. 专利号: ZL201710176527.3, 授权公告日: 2020.01.21.
[19] 姚斌;罗会颖;游帅;石鹏君;黄火清;王亚茹;柏映国;杨培龙;马锐;孟昆. 一种酸性高比活葡聚糖酶GLU16-3及其基因和应用. 专利号: ZL 201410598371.4, 授权公告日: 2017.01.11.
[20] 姚斌;罗会颖;游帅;石鹏君;黄火清;王亚茹;柏映国;杨培龙;孟昆;师霞. 一种酸性葡聚糖酶GLU16-8及其基因和应用. 专利号: ZL201410597169.X, 授权公告日: 2017.06.27.