饲料用酶制剂开发与酶工程、蚕桑资源综合利用
发表论文:
[1]Shuai You, Yi-Xin Zhang, Fan Shi, Wen-Xin Zhang, Jing Li, Sheng Zhang, Zhong-Li Chen, Wei-Guo Zhao, Jun Wang*. Lowering energy consumption for fermentable sugar production from Ramulus mori: Engineered xylanase synergy and improved pretreatment strategy. Bioresour Technol. 2022, 344(Pt B):126368.
[2]Shuai You, Jing Li, Fang Zhang, Zhi-Yuan Bai, Saidi Shittu, Richard-Ansah Herman , Wen-Xin Zhang, Jun Wang*. Loop engineering of a thermostable GH10 xylanase to improve low-temperature catalytic performance for better synergistic biomass-degrading abilities. Bioresour Technol. 2021, 342:125962.
[3] Shuai You, Ziqian Zha, Jing Li, Wenxin Zhang, Zhiyuan Bai, Yanghao Hu, Xue Wang, Yiwen Chen, Zhongli Chen, Jun Wang* and Huiying Luo*. Improvement of XYL10C_ΔN catalytic performance through loop engineering for lignocellulosic biomass utilization in feed and fuel industries. Biotechnol Biofuels, 2021, 14:195.
[4]Shuai You, Chen Xie, Rui Ma, Huo-qing Huang, Richard Ansah Herman, Xiao-yun Su, Yan Ge, Hui-yi Cai, Bin Yao, Jun Wang* and Hui-ying Luo*. Improvement in catalytic activity and thermostability of a GH10 xylanase and its synergistic degradation of biomass with cellulase. Biotechnol Biofuels. 2019, 12:278.
[5] Shuai You, Chun-Chi Chen, Tao Tu, Xiaoyu Wang, Rui Ma, Hui-yi Cai, Rey-Ting Guo*, Hui-ying Luo* and Bin Yao*. Insight into the functional roles of Glu175 in the hyperthermostable xylanase XYL10C-ΔN through structural analysis and site-saturation mutagenesis. Biotechnol Biofuels. 2018, 11:159.
[6]Shuai You, Tao Tu, Lujia Zhang, Yuan Wang, Huoqing Huang, Rui Ma, Pengjun Shi, Yingguo Bai, Xiaoyun Su, Zhemin Lin, Huiying Luo* and Bin Yao*. Improvement of the thermostability and catalytic efficiency of a highly active β-glucanase from Talaromyces leycettanus JCM12802 by optimizing residual charge-charge interactions. Biotechnol Biofuels. 2016, 9:124.
[7]Shuai You, Tao Tu, Rui Ma, Huo-qing Huang, Yuan Wang, Ying-guo Bai, Xiao-yun Su, Hui-yi Cai, Bin Yao,* and Hui-ying Luo*. Functional Analysis of a Highly Active β‑Glucana. J Agric Food Chem. 2018, 66(37):9728-9737.
[8]Fan Zhang,Shuai You, Ting Huang, Jin-Zheng Wang , Lin-Lin Zhu, Bo Wang, Wang-Sheng Ye, Richard Ansah Herman, Heng Luo, Jun Wang⁎. Dual promoter strategy enhances co-expression of α-L-rhamnosidase and enhanced fluorescent protein for whole-cell catalysis and bioresource valorization. Science of the Total Environment. 2020, 722:137865.
[9]Zhao-Xin Liu,Shuai You, Bin-Ping Tang, Bo Wang, Sheng Sheng, Fu-An Wu, Jun Wang. Inositol as a new enhancer for improving lipid production and accumulation in Schizochytrium sp. SR21. Environmental Science and Pollution Research. 2019, 26(35):35497-35508.
[10] Zi‑Qian Zha, Shuai You, Yang‑Hao Hu, Fang Zhang, Yi‑Wen Chen, Jun Wang. Asn57 N‑glycosylation promotes the degradation of hemicellulose by β‑1,3–1,4‑glucanase from Rhizopus homothallicus. Environmental Science and Pollution Research. 2022:1-15.
[11]Jin-Zheng Wang,Cheng-Kun Wu, Cheng-Hai Yan, Huan Chen, Shuai You, Sheng Sheng, Fu-An Wu and Jun Wang *. Nutritional targeting modification of silkworm pupae oil catalyzed by a smart hydrogel immobilized lipase. Food Funct. 2021, 12:6240.
[12]Cheng-Hai Yan,Xiao-Meng Xun, Jiao Wang, Jin-Zheng Wang, Shuai You, Fu-An Wu and Jun Wang *. An alternative solution for α-linolenic acid supplements: in vitro digestive properties of silkworm pupae oil in a pH-stat system. Food Funct. 2021, 12:2428.
[13] Can‑Yang Shi,Yan Hu, Ya‑Li Chai, Cheng‑Kun Wu, Ji‑Long Wu, Shuai You, Jun Wang. Kitchen Waste Oil Convert to Biodiesel via W/O Interface Biocatalysis with Thermomyces Lanuginosus Lipase–PNIPAAm Conjugates. Waste and Biomass Valorization. 2022, 13:3945–3956.
授权/申请发明专利:
[1] 游帅;张温馨;查子千;谢晨;葛研;胡洋浩;王俊;木聚糖酶突变体及其制备方法与应用. 国家发明专利证书: ZL 202110178825.2, 授权公告日: 2021.08.10.
[2]游帅;葛研;谢晨;查子千;王俊;一种40°C下高比活木聚糖酶突变体及其构建方法与应用. 国家发明专利证书: ZL 201910970877.6, 授权公告日: 2021.08.27.
[3] 游帅;张温馨;谢晨;查子千;葛研;王俊;一种GH16家族耐热葡聚糖酶突变体及其构建方法与应用. 国家发明专利证书: ZL 202011456604.9, 授权公告日: 2021.11.09.
[4] 游帅;王俊;谢晨;朱林琳;盛晟;吴福安;一种高催化效率木聚糖酶突变体及其构建方法与应用. 国家发明专利证书: ZL 201910659589.9, 授权公告日: 2019.07.22.
[5] 葛研;游帅;谢晨;查子千;王俊;动物体温下高比活耐热木聚糖酶突变体及其应用. 国家发明专利证书: ZL 201910659589.9, 授权公告日: 2019.07.22.
[6] 游帅;陈忠立;庄愉;储呈平;葛研;王俊;一组GH10家族耐高温木聚糖酶突变体及其应用. 国家发明专利证书: ZL 202110854745.4, 授权公告日: 2022.07.22.
[7] 游帅;谢晨;张温馨;张访;陈奕文;胡洋浩;王俊;组GH16家族耐热β-1, 3-1, 4-葡聚糖酶突变体及其应用. 国家发明专利证书: ZL 202110623476.0, 授权公告日: 2022.07.22.
[8] 游帅;查子千;谢晨;张温馨;张访;王雪;陈奕文;王俊;一组GH16家族葡聚糖酶的N-糖基化突变体及其应用. 国家发明专利证书: ZL202110621950. 6, 授权公告日: 2022.07.22.
[9] 王俊;里查德·安萨·赫尔曼;游帅;白致远;一种生产重组天冬氨酸蛋白酶的工程菌及其应用. 国家发明专利证书: ZL 202110623445. 5, 授权公告日: 2022.07.22.
[10] 游帅;张温馨;查子千;谢晨;葛研;胡洋浩;王俊;木聚糖酶突变体及其制备方法与应用. 国家发明专利证书: ZL 202110178825.2, 授权公告日: 2021.08.10.
[11] 姚斌;罗会颖;游帅;王苑;涂涛;黄火清;苏小运;柏映国;王亚茹;一种木聚糖酶突变体及其制备方法和应用. 国家发明专利证书: ZL 201710176527.3, 授权公告日: 2020.01.21.
[12] 姚斌;罗会颖;游帅;石鹏君;黄火清;王亚茹;柏映国;杨培龙;马锐;孟昆;一种酸性高比活葡聚糖酶GLU16-3及其基因和应用. 国家发明专利证书: ZL 201410598371.4, 授权公告日: 2017.01.11.
[13] 姚斌;罗会颖;游帅;石鹏君;黄火清;王亚茹;柏映国;杨培龙;孟昆;师霞;一种酸性葡聚糖酶GLU16-8及其基因和应用. 国家发明专利证书: ZL 201410597169. X, 授权公告日: 2017.06.27.
[14] 游帅;葛研;谢晨;张温馨;王雪;陈奕文;张伊欣;王俊;一组动物体温下高催化活力β-1,3-1,4-葡聚糖酶突变体及其应用. 国家发明专利申请公开号: CN113699136A, 申请公布日: 2021.11.26.
[15] 游帅;胡洋浩;赵卫国;王俊;陈奕文;李龙;一种耐热木聚糖酶突变体及其应用. 国家发明专利申请公开号: CN113862243A, 申请公布日: 2021.12.31.
[16] 游帅;张晟;胡洋浩;张访;张温馨;陈奕文;王俊;一种高比活木聚糖酶突变体及其应用. 国家发明专利申请公开号:CN114107262A, 申请公布日: 2022.03.01.
[17] 游帅;张温馨;陈忠立;周春生;储呈平;孙道权;王俊;一种热稳定性提高的葡聚糖酶突变体及其应用. 国家发明专利申请公开号:CN114317495A, 申请公布日: 2022.04.12.
[18] 游帅;张温馨;陈忠立;周春生;储呈平;孙道权;王俊;一种葡聚糖酶突变体及其应用. 国家发明专利申请公开号: CN114381448A, 申请公布日: 2022.04.22.
[19] 游帅;张温馨;陈忠立;周春生;储呈平;赵卫国;王俊;热稳定性提升的酸性葡聚糖酶突变体及其应用. 国家发明专利申请公开号: CN114517191A, 申请公布日: 2022.05.20.
[20] Shuai You; Zhongli Chen; Yu Zhuang; Chengping Chu; Yan Ge; Jun Wang. Group of high temperature resistant xylanase mutants of GH10 family and application thereof. PCT专利申请,PCT/CN 2021/113743. 国际公布日:2021.09.15.
[21] Shuai You; Wenxin Zhang; Chen Xie; Yan Ge; Yanghao Hu; Jun Wang. Xylanase mutant and its preparation method and application. 美国发明专利, Application Number: 17/219550.2021.03.31.
[22] Jun Wang; Shuai You; Linlin Zhu; Yang Li; Weidong Hao; Litian Geng; Yiyun Kuang; Yuxiao Chang. Truncation mutant of α-L-rhamnosidase and application thereof. PCT专利申请, PCT/CN 2021/073707.国际公布日:2021.11.25